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Specific fluorescence-tagged substrates for each proteasomal β subunits (Enzo Life Sciences, NY, USA): Z-LLE-AMC for β1, Boc-LSTR-AMC for β2 and Suc-LLVY-AMC for β5 were used to initiate the reaction [27–30]

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The roots exit through their respective neural foramina, and travel between the anterior and middle/posterior scalene muscles in the interscalene triangle, or space (Figures 2, 3)