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A critical obstacle in the development of CNS therapies is the blood–brain barrier (BBB), initially identified by Paul Ehrlich in the late 19th and early 20th centuries

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This is in part owing to more challenging targets (large protein complexes, cell-surface receptors and membrane proteins) where protein expression is carried out in eukaryotic systems and selenomethionine labelling is problematic (Rose & Wang, 2016 ▸)