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Following 30 s of baseline measurement, aliquots of 1 million cells/mL were then stimulated for 210 s at RT with either 20 μg/mL of UKNP4.1.1 E2E1-I53-50A trimers, 25 μg/mL of UKNP4.1.1 E2E1-NP or mosaic E2E1-NP (i.e., equimolar trimer)