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Using a multiple-sequence alignment of all 279 GUS enzymes identified from the HMP, we found that 14 contained a loop insert of roughly 25 residues, like in Eubacterium eligens GUS (6BJW) (29), that were near the active site, adjacent to the Loop 1/mini-Loop 1 site, that could potentially contribute to binding (Fig

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Some practitioners suggest cycling, with a 6-12 week usage period followed by a 4-8 week break between cycles