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Specific binding was plotted as a function of ligand concentration, and a single-site binding equation of the form y = B max × x /( K d + x ) was fitted to the data, where B max is the maximum saturable binding and K d is the equilibrium dissociation constant

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doi: 10.1093/humrep/dez001 [DOI] [PubMed] [Google Scholar] 23.Yang W, He H, Wang T, et al