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The pathogenic variants in ZBTB24, CDCA7, and HELLS disrupt DNA methylation maintenance unrelated to replication, leading to low methylation in specific regions that rely on CDCA7/HELLS complexes for chromatin remodeling (79)

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Side Effects & Safety Reported in clinical series (injection-based protocols): injection site reactions (mild erythema, most common), transient headache, mild fluid retention at initiation