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Under anesthesia, opened the abdominal cavity and exposed liver, inserted a catheter into the superior vena cava and perfused liver with buffer I (D’Hanks containing 180 mg/L EGTA and 80 mg/L NaOH) at a flow rate of 4.0 mL/min, followed by buffer II (D’Hanks containing 1 g/L collagenase IV and 444 mg/L CaCl2) at a flow rate of 2.0 mL/min

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Safety Classification: - For use by qualified research personnel only