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Quantification of the number of total lysosomes (j) and the number of lysosomes with different (indicated) diameters (k-m) in control, shITM2C#1 or shITM2C#1 + ITM2C(RR)-FLAG HeLa cells under N or H–R (n = 53 cells for control-N and control-H–R, n = 49 cells for shITM2C-N, n = 45 cells for shITM2C-H–R, n = 48 cells for other cells, three experiments)