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The protein was eluted by a linear gradient against 500 mM imidazole (elution peak around 150 mM imidazole)

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Studies have also shown that LPS can induce a structurally unique self-replicating α-Syn fibril strain in mice, which triggers a signature pattern of synucleinopathy similar to that induced by wild-type α-Syn commonly observed in PD (57)