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Among the tested alkaloids, 7-hydroxymitragynine had the highest and selective affinity for hMOR with a K i value of 47 nM, followed by mitragynine (K i = 233 nM), paynantheine (K i = 410 nM), speciociliatine (K i = 560 nM), and speciogynine (K i = 728 nM)