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A UV detector at the end of the column measures the absorbance of the eluting compounds at a specific wavelength (usually 214-220 nm, where the peptide backbone bond absorbs light)

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Standard reconstitution targets 1–2 mg/mL for most research peptides, allowing injection volumes of 0.1–0.5 mL for SubQ or 0.5–1.0 mL for IM