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The fragmented poly(A) RNA enriched samples were subjected to cDNA synthesis using Illumina TruSeq preparation kit

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Oncolytic viruses (such as T-VEC) selectively lyse tumor cells to release DAMPs (such as CRT, HMGB1) and viral nucleic acids, triggering strong immunogenic cell death (ICD) and activating the cGAS-STING pathway, inducing a “viral mimic” state to enhance antitumor immunity (Mohseni et al., 2021)