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Several groups have used AuNP-based immunosensors to detect AFP by immobilizing AFP antibodies onto AuNP-decorated thionine/nafion membranes, 789,790 through electro-deposition of AuNPs and prussian blue on ITO electrode, 791 using carbon paste electrode constructed with an ionic liquid and AuNPs, 792 with AFP-antibody functionalized AuNPs, 793 using chitosan-AuNP composite film on a GCE, 794 using films of MWCNT/DNA/thionine/AuNPs, 795 using 1,1′-bis-(2-mercapto)-4,4′-bipyridinium dibromide functionalized AuNPs onto GCE, 796 and by immobilizing AFP-antigen onto GCE modified with AuNPs and a CNT doped chitosan film