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LC-MS/MS identification of all pulled-down proteins Bioinformatic filtering against negative controls Functional annotation and pathway enrichment Ideal for unbiased interaction discovery Applications Interaction validation : Confirm protein-protein interactions discovered by Y2H, phage display, or computational prediction using a purified, biochemical system Domain mapping : Identify which structural domains or amino acid residues are required for interaction using deletion mutants and point variants as bait Interaction specificity profiling : Test a bait protein against panels of related prey proteins to characterize binding selectivity Novel interaction discovery : Screen cell lysates or cDNA expression libraries to identify previously unknown binding partners Competition assays : Evaluate whether small molecules, peptides, or competing proteins disrupt a specific interaction Post-translational modification dependence : Determine whether phosphorylation, acetylation, or other modifications are required for interaction by comparing modified and unmodified bait proteins Why Choose Profacgen
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