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Evaluation of In Vitro Methionine γ-Lyase Cytotoxicity on HT29 Cell Line by MTS Assay and Trypan Blue Exclusion Assay HT29 cells were treated with MGL at IC 50 (0.03 ± 0.001 mg/ml, Results Section) and grown in DMEM supplemented with 5% FBS, 1% L-glutamine, and 1% penicillin/streptomycin, at 37°C, 5% CO 2 , for 24, 48, and 72 h

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2022;156:105071 [CrossRef] [Google Scholar] Progression to Diabetes in Relatives of Type 1 Diabetic Patients: Mechanisms and Mode of Onset