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Hence, replacement of cysteine with SeCys is detrimental to protein structure and function as SeCys is larger, reactive and more easily deprotonated than cysteine (Hondal et al., 2012), as seen in case of methionine sulfoxide reductase function that got impaired after substitution with SeCys (Châtelain et al., 2013)
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As hypothesized, the 18 O content in cyanidin ( 8 ) increased as a function of equilibration time and the expected level (47.4%) for stochastic incorporation of a single 18 O label was reached after 15 min of pretreatment (Fig
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