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Starting on day 22 post-injection, mice were administered 20 mg/kg BW curcumin via oral gavage 49 once daily, or 50 mg/kg BW gemcitabine via intraperitoneal injection twice a week 54 or the combination of curcumin and gemcitabine

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CM-H 2 DCF-DA, nonyl acridine orange (NAO), Rhodamine-123 and mounting medium, supplemented with 4′,6-diamidino-2-phenylindole (DAPI), were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA)