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Interestingly, the effects of NO were demonstrated to be biphasic in vitro, where low levels of NO donor glyceryl trinitrate (10 μM and 50 μM) induced the expression of adhesion molecules in HUVECs in an NF-κB-dependent manner, whereas higher levels (250 μM and 500 μM) were suppressive (Sektioglu, Carretero et al