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Peptides were separated using a C18 analytical column (nanoEase M/Z HSS C18 T3 (75 μm by 25 cm, 100 Å, Waters) with a 150-min run, comprising three consecutive steps with linear gradients from 3 to 35% B in 120 min, from 35 to 50% B in 5 min, from 50 to 85% B in 2 min, followed by isocratic elution at 85% B in 5 min and stabilization to initial conditions [A: 0.1% formic acid (FA) in water

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The molecule is universal and is synthesized in most organs particularly the liver and found throughout the tissue in mammals