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Ilyas et al., 2016

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Cancer Research Senescence-associated cancer prevention – Eliminates pre-cancerous senescent cells Chemotherapy-induced senescence – Reduces side effects of cancer treatments Tumor microenvironment modulation – Improves anti-tumor immune responses FOXO4-DRI Peptide: Chemical Composition & Structure Molecular Formula: C₁₅₆H₂₄₈N₄₆O₄₆S₂ Molecular Weight: 3428.9 g/mol Amino Acid Sequence: Leu-Thr-Leu-Ser-Gln-Arg-Pro-Arg-Lys-Leu-Ala-Gly-Ala-Lys-Arg-Arg-Gln-Leu-Lys-Glu-Asn-Arg-Arg-Gln-Ile-Lys-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Glu-Glu (D-retro-inverso form) Key Structural Features: D-retro-inverso peptide – Modified peptide backbone for enhanced stability and resistance to degradation FOXO4-p53 interaction domain – Mimics the FOXO4 protein region that binds to p53 Highly selective binding – Specifically targets senescent cells without affecting healthy cells Small peptide size – Allows for efficient cellular uptake and tissue penetration Chemical Properties: How FOXO4-DRI Works: Mechanisms of Action FOXO4-DRI exerts its effects by disrupting the interaction between FOXO4 and p53 , a critical pathway that prevents senescent cells from undergoing apoptosis