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We thank the former Eaton lab, Robert Tjian and Lina Partridge for providing antibodies as well as Mike Henne, Seung Kim, Samuel Liegeois, Ronald Kühnlein, Yohanns Bellaiche, Bruno Lemaitre, the Bloomington stock center, FlyORF and Vienna Drosophila RNAi Center (VDRC) for providing flies

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Results Disruption of the glucagon receptor significantly alters both gene expression and the interactive network of α cells To comprehensively examine the functional and physiological changes in α cells following GCGR blockade, we conducted single-cell RNA sequencing (scRNA-seq) on islets isolated from 12–14-week-old male wild-type (WT) and GCGR-knockout (KO) mice (Fig