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PCR assays PCR assays for confirmation of the identified bacteria and determination of antibiotic resistance genes Since polymerase chain reaction (PCR) and quantitative PCR (qPCR) are very sensitive for detecting total DNA present in a sample, including DNA from live and dead cells 19 and due to process-related DNA changes, PCR-based approaches allow the direct identification of DNA damage during disinfection 31

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Targeted disruption of the mouse lysosomal acid lipase gene: long-term survival with massive cholesteryl ester and triglyceride storage

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