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Then, 200 μL of DCFH-DA working solution was added to each well, and the cells were incubated at 37 °C for 30 min in the dark

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Although, Allum sativa extract is rich with direct antioxidants, studies demonstrated its potent ability to induce the endogenous antioxidant machinery of Nrf2 by enhancing its phosphorylation (by PKC and p38 MAPK) and decreasing its ubiquitination (Capasso, 2013)