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The disappearance of mutants from calf tissue was in agreement with the results of an ex-vivo viability assay, where wild-type MAP survival was also hindered by effector immune responses in bovine macrophages alone and macrophages incubated with unprimed PBMCs in both vaccine groups

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Berberine and its more biologically available derivative, dihydroberberine, inhibit mitochondrial respiratory complex I