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Mechanisms of alveolar type 2 epithelial cell death during acute lung injury

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Chromatin immunoprecipitation assay MEFs( Keap −/− , Nrf2 +/+ , and Nrf2 −/− )were harvested and the chromatin immunoprecipitation(CHIP)assay was performed using a commercially available kit (Upstate Biotechnology, Lake Placid, NY, USA)